บทความวิจัย/วิชาการ
Scopus
Current Traditional Medicine
2569
การพัฒนาอุปกรณ์สกัดสารสำคัญจากพืชด้วยกระแสไฟฟ้า
Pulse Electric Field Assisted Extraction of Pinostrobin and Panduratin a From Boesenbergia Rotunda (L.) Mansf. and Biological Assessment on Antioxidation And Glucose Uptake Inhibition
Abstract: Background: Boesenbergia rotunda (L.) Mansf., a medicinal plant from the Zingiberaceae
family native to tropical regions, is valued for its bioactivity properties, commonly used as
both a food ingredient and a traditional health tonic remedy. Although it has well-recognized potential
as a material source of health products, research on the extraction method for bioactive enrichment
remained unexplored.
Objective: To enhance the extraction process and bioactive content of the plant by using pulse
electric field (PEF) extraction compared to the classical extraction method.
Methods: Fresh rhizomes were macerated in 95% ethanol for 7, 15, 30, and 60 days for classical
extraction. For PEF extraction, 95% ethanol was used as the extraction solvent with time variations
of 30 to 150 minutes at a pulse rate of 116 pulses/min and a frequency of 50 Hz. The extraction
efficiency was assessed by measuring bioactive compounds, pinostrobin (PS) and panduratin
A (PA) using an HPLC assay, along with total flavonoid and phenolic content. Antioxidant activities
were evaluated via DPPH, FRAP, and ABTS assays, while glucose uptake inhibition was tested
by using a yeast cell model.
Results: Classical extraction achieved PS and PA equilibrium after 15 days, recovery of 937-970
mg/kg (0.093-0.097%w/w) and 26-28 mg/kg (0.0026-0.0028%w/w), respectively. PEF extraction
optimized at 60 minutes significantly improved recovery with PS at 930±20.35 mg/kg and PA
at143.4±3.48 mg/kg (0.014±0.0003%w/w). Additionally, PEF extraction also increased phenolic
and flavonoid contents, antioxidant activity, and glucose uptake inhibition.
Conclusion: PEF extraction presents a promising method for efficiently extracting bioactive compounds
from Boesenbergia rotunda.
Pulse electric field, Boesenbergia rotunda (L.) Mansf., pinostrobin, panduratin A, glucose uptake inhibition, antioxidation.
https://www.benthamscience.com/public/journals/current-traditional-medicine
https://www.benthamscience.com/public/journals/current-traditional-medicine